16s RNA from Rust at ascidian/steel interface - SRA

SRX16983079: 16s RNA from Rust at ascidian/steel interface
1 ILLUMINA (Illumina NovaSeq 6000) run: 84,127 spots, 35.1M bases, 11Mb downloads

Design: DNA extracted from sediment and rust were used for sequencing. The integrity and approximate length of the DNA were examined by 1% agarose gel electrophoresis. For Illumina HiSeq sequencing, 16S rRNA genes from bacteria and eukarya were amplified using the primer sets 515F/806R and 528F/706R, respectively. PCR products were purified with a PCR purification kit (QIAGEN, Hilden, NRW, Germany). The PCR libraries were conducted using TruSeq DNA PCRFree specimen Preparation Kit (Illumina, San Diego,CA). After quantification with Qubit, the PCR libraries were sequenced on the Illumina NovaSeq6000 platform.

Submitted by: IOCAS

Study: Microbial communities at macrofouling/steel interface

PRJNA865886 • SRP390485 • All experiments • All runs

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Investigating the effect of the microbial community at the macrofouling/steel interface on steel corrosion

Sample: Rust at ascidian/steel interface

SAMN30139941 • SRS14568957 • All experiments • All runs

Organism: steel metagenome

Library:

Name: AS.RU3

Instrument: Illumina NovaSeq 6000

Strategy: AMPLICON